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This study evaluates the sublethal effects of deltamethrin on Cytochrome P450 (CYP450) levels and EROD (ethoxyresorufin-O-desethylase) enzyme activity in Ancistrus multispinnis. Deltamethrin, a type II pyrethroid insecticide, is widely used in agriculture and is highly toxic to aquatic organisms. Fish of the species Ancistrus multispinnis were exposed to sublethal doses of 3 and 4 mg/kg of deltamethrin, administered intracoelomatically. After 96 hours, liver samples were collected for biomarker analysis. The results indicated a significant increase in total CYP450 levels and EROD activity in fish exposed to deltamethrin, especially at a dose of 4 mg/kg. Statistical analysis confirmed that these differences were highly significant (p < 0.01). The induction of CYP450 and EROD suggests that deltamethrin activates specific metabolic pathways related to xenobiotic detoxification. Exposure to deltamethrin can increase the production of reactive oxygen species (ROS), resulting in oxidative stress in fish. ROS production is a common byproduct of CYP450 and EROD-induced metabolic pathways, contributing to the compound's overall toxicity. These findings reinforce the importance of continuous monitoring of the presence of deltamethrin in aquatic environments and the application of biomarkers such as CYP450 and EROD in environmental biomonitoring programs.
References
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